High Throughput Screening (HTS) Assays for DNA Topoisomerases

​Supercoiling-Dependent Fluorescence Quenching (SDFQ) HTS Assays

SDFQ HTS assay kits

• SDFQ HTS assay kits for E. coli DNA gyrase: relaxed pAB1_FL905 and pAB1_FL924; 5 x gyrase buffer; E. coli DNA gyrase.
• SDFQ HTS assay kits for Mtb DNA gyrase: relaxed pAB1_FL905 and pAB1_FL924; 5 x gyrase buffer; Mtb DNA gyrase.
• SDFQ HTS assay kits for E. coli DNA topoisomerase I: supercoiled pAB1_FL905 and pAB1_FL924; 10 x TopoI buffer; E. coli DNA topoisomerase I.

Enzymes and plasmids

• Relaxed plasmid pAB1_FL905.
• Relaxed plasmid pAB1_FL924.
• Supercoiled plasmid pAB1_FL905.
• Supercoiled plasmid pAB1_FL924.
• E. coli DNA gyrase.
• Mtb DNA gyrase.
• E. coli DNA topoismerase I.
• Variola DNA topoisomerase I.