HTS assays for Discovering Bacterial DNA Gyrase Poisons

Gyrase poison HTS assay kits

• Gyrase poison HTS kits for E. coli DNA gyrase: plasmid pBR322; 5 x gyrase buffer; E. coli DNA gyrase, 2.5% sarkasyl, proteinase K, T5 exonuclease, and SYBR Green.
• Gyrase poison HTS kits for Mtb DNA gyrase: plasmid pBR322; 5 x gyrase buffer; Mtb DNA gyrase, 2.5% sarkasyl, proteinase K, T5 exonuclease, and SYBR Green.​

Enzymes and plasmids

• Plasmid pBR322.​
  
• E. coli DNA gyrase.
• Mtb DNA gyrase.
• T5 exonuclease.