Synthetic DNA Circle Production
Recently, a novel biochemical method was established to synthesize supercoiled, double-stranded circular DNA molecules—referred to as Synthetic DNA Circles (SDCs)—using purified enzymes. This in vitro method is highly scalable, capable of producing pure supercoiled SDCs in quantities ranging from micrograms to milligrams and beyond.
The resulting DNA molecules are entirely free from genomic DNA, RNA, endotoxins, and antibiotic contaminants. Furthermore, SDCs do not contain unnecessary bacterial sequences, such as origins of replication (ori) or antibiotic resistance genes.
Product Proof-of-Concept
To date, we have synthesized approximately one milligram of a 2,002 bp supercoiled SDC (EGFP-FL). This construct carries the EGFP gene and all necessary components for expression in human cells, completely devoid of bacterial DNA elements. Notably, it lacks epigenetic modifications typical of bacterial production, such as Dam and Dcm methylation.
Intellectual Property and Publication
This technology was developed in Professor Leng’s laboratory at Florida International University. The research is detailed in the following peer-reviewed publication in Nucleic Acids Research:
Rezaei S, Moncada-Restrepo M, Leng S, Chambers JW, Leng F. Synthesizing supercoiled circular DNA molecules in vitro. Nucleic Acids Res. 2025;53:gkaf889.
This technology is protected by the following patent: US12371725B2
If interested in this technology, please contact us ([email protected] or [email protected]).
The resulting DNA molecules are entirely free from genomic DNA, RNA, endotoxins, and antibiotic contaminants. Furthermore, SDCs do not contain unnecessary bacterial sequences, such as origins of replication (ori) or antibiotic resistance genes.
Product Proof-of-Concept
To date, we have synthesized approximately one milligram of a 2,002 bp supercoiled SDC (EGFP-FL). This construct carries the EGFP gene and all necessary components for expression in human cells, completely devoid of bacterial DNA elements. Notably, it lacks epigenetic modifications typical of bacterial production, such as Dam and Dcm methylation.
Intellectual Property and Publication
This technology was developed in Professor Leng’s laboratory at Florida International University. The research is detailed in the following peer-reviewed publication in Nucleic Acids Research:
Rezaei S, Moncada-Restrepo M, Leng S, Chambers JW, Leng F. Synthesizing supercoiled circular DNA molecules in vitro. Nucleic Acids Res. 2025;53:gkaf889.
This technology is protected by the following patent: US12371725B2
If interested in this technology, please contact us ([email protected] or [email protected]).
Strategy to synthesize supercoiled (Sc) Synthetic DNA Circles in vitro
EGFP-FL is a SDC to express enhanced GFP in mammalian cells
Transfection efficiency of SDC is much higher than that of minicircles
Transfection efficiency of SDC is much higher than that of plasmids
Transfection efficiency of SDC is much higher than that of plasmids:
Mouse myoblast C2C12 cells
