Description: Supercoiled Plasmid pAB1 (2,757 bp) was constructed by inserting a 95 bp synthetic DNA fragment FL_AT42 between the SphI and BamHI sites of pUC18, and purified from overnight culture of E. coli Top10 strain carrying plasmid pAB1. The supercoiling density is estimated to be approximately -0.06.
Concentrations: Two concentrations of supercoiled pAB1 are available: 0.2 mg/mL and 1 mg/mL.
Shipping and storage: Supercoiled pAB1 is shipped in storage buffer (10 mM Tris HCl, pH8.0 at 25 °C) on ice and should be stored in a -20 or -80°C freezer (-70-80°C) and is stable for at least 12 months in this condition.
Concentrations: Two concentrations of supercoiled pAB1 are available: 0.2 mg/mL and 1 mg/mL.
Shipping and storage: Supercoiled pAB1 is shipped in storage buffer (10 mM Tris HCl, pH8.0 at 25 °C) on ice and should be stored in a -20 or -80°C freezer (-70-80°C) and is stable for at least 12 months in this condition.
This product is used for research only.
References
References
- Gu M, Berrido A, Gonzalez WG, Miksovska J, Chambers JW, Leng F. Fluorescently labeled circular DNA molecules for DNA topology and topoisomerases. Sci Rep. 2016 6:36006. doi: 10.1038/srep36006. PMID: 27796331; PMCID: PMC5087112.