Description: E. coli DNA gyrase fluorescence assay kit is designed for assaying bacterial DNA gyrase activities and also for drug discovery research targeting bacterial DNA gyrase. This kit includes enough enzymes and other reagents for 100 assays: E. coli DNA gyrase (100 units, Cat. No. TB-E-001) and 5xDNA gyrase assay buffer (Cat. No. TB-B-001), DNA gyrase dilution buffer (Cat. No. TB-B-002), T5 exonuclease (Cat. No. TB-E-002, 200 uL of 5 uM) and 10xT5 exonuclease assay buffer (Cat. No. TB-B-003), relaxed pAB1 (100 uL of 0.2 mg/mL relaxed pAB1, Cat. No. TB-D-001), and 40xSYBR Green. The strategy of this DNA gyrase relaxation assay is shown in one of the three figures. Please see reference 2 for details.
Storage and shipping: E. coli DNA gyrase kit is shipped with dry ice and should be stored in a -80°C freezer (-70-80°C), and is stable for at least 12 months in this condition. E. coli DNA gyrase and T5 exonuclease can be aliquoted on first thawing to prevent loss of activities from freeze-thaw cycles.
Storage and shipping: E. coli DNA gyrase kit is shipped with dry ice and should be stored in a -80°C freezer (-70-80°C), and is stable for at least 12 months in this condition. E. coli DNA gyrase and T5 exonuclease can be aliquoted on first thawing to prevent loss of activities from freeze-thaw cycles.
This product is used for research only.
E. coli DNA Gyrase Fluorescence Assay Instruction: