Synthetic DNA Circle Service

Step 1:  Synthetic DNA Circle design.
Step 2: Producing Synthetic DNA Circles.
Step 3: Quality control.
Step 4: Delivery.
Contact us at [email protected]

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​What is Synthetic DNA Circle (SDC)?

​Synthetic DNA Circles (SDCs) are double-stranded DNA molecules, synthesized using in vitro biochemical methods with purified enzymes.

​Compared to conventional plasmids and minicircles, Synthetic DNA Circles provide improved transgene expression, reduced immunogenicity, and superior molecular uniformity for advanced research and pre-clinical development.

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Our Patented Technology

Our CRO services are built on proprietary technologies protected under US Patent US12371725B2.
These methods enable the efficient in vitro synthesis of supercoiled Synthetic DNA Circles directly from linear precursors, without bacterial fermentation.
The approach has been experimentally validated and peer-reviewed in Nucleic Acids Research.

​Cell-Free Production

Fully enzymatic workflows reduce host-derived contaminants and enable precise control over DNA topology and purity.

High Supercoiling Efficiency

Optimized processing yields highly supercoiled Synthetic DNA Circles suitable for transfection and delivery studies.

Pre-clinical R&D Focus

Designed to support discovery research and pre-clinical R&D programs without regulatory over-commitment.

Synthetic DNA Circles Production Workflow

1. Custom DNA sequence design
2. Linear DNA generation (PCR or RCA)
3. Intramolecular circularization
4. Selective removal of linear DNA
5. Supercoiling and final purification

Key Advantage: Our bacterial-free workflow enables rapid turnaround, reduced variability, and consistent Synthetic DNA Circles quality for research and pre-clinical studies.

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Applications

• Gene therapy and DNA vaccination research
• CRISPR and genome editing delivery
• Cell reprogramming and synthetic biology
• Regulatory element and promoter studies
• DNA nanotechnology and diagnostic development

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​Scientific Validation

Our technologies are supported and protected by issued patents and peer-reviewed publications:

• US12371725B2 — Methods for synthesizing circular DNA molecules in vitro
• Nucleic Acids Research (2025): Synthesizing supercoiled circular DNA molecules in vitro

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​Contact Us

To discuss your research or pre-clinical R&D project, please contact us:
📧 [email protected]