T5 Exonuclease (T5E) AT-hairpin-based HTS Assays

​T5E AT-hairpin-based HTS kits

• T5E AT-hairpin-based HTS kits for E. coli DNA gyrase: relaxed pAB1; 5 x gyrase buffer; E. coli DNA gyrase, T5 exonuclease, and SYBR Green.
• T5E AT-hairpin-based HTS kits for Mtb DNA gyrase: relaxed pAB1; 5 x gyrase buffer; Mtb DNA gyrase, T5 exonuclease, and SYBR Green.
• T5E AT-hairpin-based HTS kits for E. coli DNA topoisomerase I: supercoiled pAB1; 10 x TopoI buffer; E. coli DNA topoisomerase I, T5 exonuclease, and SYBR Green.
• T5E AT-hairpin-based HTS kits for DNA intercalators: supercoiled pAB1; 10 x buffer, T5 exonuclease, and SYBR Green.

Enzymes and plasmids

• Relaxed plasmid pAB1.
• Supercoiled plasmid pAB1.​
• E. coli DNA gyrase.
• Mtb DNA gyrase.
• E. coli DNA topoismerase I.
• Variola DNA topoisomerase I.